Paul Scott "Paco" Lockhart is an American aerospace engineer, retired United States Air Force Colonel and NASA astronaut, a veteran of two Space Shuttle missions.

Christopher Joseph "Gus" Loria is a retired United States Marine Corps Colonel and a medically retired NASA astronaut. He was originally scheduled to fly on STS-113 as pilot; however, he was grounded from spaceflight due to a severe back injury.

William Cameron "Willie" McCool was an American naval officer and aviator, test pilot, aeronautical engineer, and NASA astronaut, who was the pilot of Space Shuttle Columbia mission STS-107. He and the rest of the crew of STS-107 were killed when Columbia disintegrated during re-entry into the atmosphere. He was the youngest male member of the crew. McCool was posthumously awarded the Congressional Space Medal of Honor.

Mark Lewis "Roman" Polansky is an American aerospace engineer and research pilot and a former NASA astronaut. Polansky received the nickname "Roman" as a joke, because he shares a last name with director Roman Polanski. He flew on three Space Shuttle missions: STS-98, STS-116, and STS-127.

David McDowell Brown was a United States Navy captain and a NASA astronaut. He died on his first spaceflight, when the Space Shuttle Columbia (STS-107) disintegrated during orbital reentry into the Earth's atmosphere. Brown became an astronaut in 1996, but had not served on a space mission prior to the Columbia disaster. Brown was posthumously awarded the Congressional Space Medal of Honor.

Daniel Christopher Burbank is a retired American astronaut and a veteran of two Space Shuttle missions. Burbank, a Captain in the United States Coast Guard, is the second Coast Guard astronaut after Bruce Melnick. Burbank was born in Manchester, Connecticut, and raised in Tolland, Connecticut, where he graduated from Tolland High School. He attended Fairfield University his freshman year before transferring to the United States Coast Guard Academy, where he earned his commission in 1985. In 1987, he went through flight training and became an instructor pilot, serving at various Coast Guard stations at Coast Guard Air Station Elizabeth City, Coast Guard Air Station Cape Cod, and Coast Guard Air Station Sitka.

Yvonne Darlene Cagle is an American astronaut.

Fernando "Frank" Caldeiro was an Argentine-born American scientist and NASA astronaut.

Charles Joseph "Charlie" Camarda is an American engineer and a NASA astronaut who flew his first mission into space on board the Space Shuttle mission STS-114. He served as Senior Advisor for Engineering Development at NASA Langley Research Center.

Laurel Blair Salton Clark was an American medical doctor, United States Navy Captain, NASA astronaut and Space Shuttle mission specialist. Clark died along with her six fellow crew members in the Space Shuttle Columbia disaster. She was posthumously awarded the Congressional Space Medal of Honor.

Edward Michael "Mike"/"Spanky" Fincke is an American astronaut who formerly held the American record for the most time in space (381.6 days). His record was broken by Scott Kelly on October 16, 2015. He was born in Pittsburgh, Pennsylvania, but considers its suburb Emsworth to be his hometown. He is a United States Air Force officer and a NASA astronaut, and served two tours aboard the International Space Station as a flight engineer and commander. He flew on one Space Shuttle mission, STS-134 as a Mission Specialist.

Patrick Graham Forrester is a retired United States Army officer and a NASA astronaut. At the time of his retirement from the U.S. Army, Forrester had achieved the rank of colonel. He is married and has two children. Forrester has flown on three Space Shuttle missions, STS-105, STS-117 and STS-128. He is the current Chief of the Astronaut Office, having assumed the role from Chris Cassidy in June 2017.