Karen LuJean Nyberg is an American mechanical engineer and NASA astronaut. Nyberg became the 50th woman in space on her first mission in 2008. Nyberg started her space career in 1991 and spent a total of 180 days in space in 2008 and 2013 (as a Mission Specialist on STS-124 and a Flight Engineer on Soyuz TMA-09M).

Nicole Marie Passonno Stott is an American engineer and a NASA astronaut. She served as a Flight Engineer on ISS Expedition 20 and Expedition 21 and was a Mission Specialist on STS-128 and STS-133. After 27 years of working at NASA, the space agency announced her retirement effective June 1, 2015.

Randolph James "Komrade" Bresnik is an officer in the United States Marine Corps and a NASA astronaut. A Marine Aviator by trade, Bresnik was selected as a member of NASA Astronaut Group 19 in May 2004.[5] First launched to space on STS-129. Served as Flight Engineer for Expedition 52, and as ISS Commander for Expedition 53.

James Patrick "Mash" Dutton, Jr. is a NASA astronaut pilot of the Class of 2004 (NASA Group 19), and a former test pilot in the US Air Force with the rank of Colonel.

Christopher John "Chris" Cassidy is a NASA astronaut and United States Navy SEAL. Chris Cassidy achieved the rank of Captain in the U.S. Navy. He was the Chief of the Astronaut Office at NASA from July 2015 until June 2017. Cassidy attended York High School, in York, Maine. He then graduated from the Naval Academy Preparatory School in Newport, Rhode Island, in 1989. He received a Bachelor of Science degree in Mathematics from the United States Naval Academy in 1993 and a Master of Science degree in Ocean Engineering from MIT in 2000. While in the Navy, Cassidy passed BUD/S and became a Navy SEAL. While a SEAL he served several tours of duty supporting the fight in the War on Terror. His first spaceflight was on Space Shuttle mission STS-127, and his second was as a flight engineer for Expedition 35/36, launched aboard Soyuz TMA-08M. He was in space between July 15–31, 2009 and March 28 – September 10, 2013. Cassidy has worked as a CAPCOM for both International Space Station and Space Shuttle missions in the past. Cassidy announced his retirement from both the Navy and NASA Astronaut Corps on May 28, 2021.

Robert Shane Kimbrough is a retired United States Army officer, and a NASA astronaut. He was part of the first group of candidates selected for NASA astronaut training following the Space Shuttle Columbia disaster. Kimbrough is a veteran of two spaceflights, the first being a Space Shuttle flight, and the second being a 6-month mission to the ISS on board a Russian Soyuz craft. He was the commander of the International Space Station for Expedition 50 and later spent 6 months on the ISS as part of the SpaceX Crew-2 mission. He retired from NASA on July 31, 2022.

Thomas Henry "Tom" Marshburn is an American physician and a NASA astronaut. He served as a Mission Specialist on STS-127. Marshburn was a member of the Soyuz TMA-07M crew which launched to ISS in December 2012 to join Expedition 34. Served as flight engineer for Expedition 34 and 35.

Robert Lee "Bobby" Satcher Jr. is an American physician, chemical engineer, and NASA astronaut. He became the first orthopedic surgeon in space during STS-129. He participated in 2 spacewalks during STS-129, accumulating 12hrs 19min of EVA time. Satcher holds two doctorates (Ph.D., M.D.) and has received numerous awards and honors as a surgeon and engineer.

Walker served as a mission specialist during NASA’s SpaceX Crew-1 mission to the International Space Station in 2020, the first crewed operational Dragon spacecraft flight. She also was the first woman to fly aboard a Dragon spacecraft. Once aboard the orbiting laboratory, Walker joined the Expedition 64/65 crew and briefly commanded Expedition 65, logging 167 days in space before returning to Earth in May 2021. She spent 163 days in space during her first spaceflight in 2010 as a member of the space station’s Expedition 24/25 crew. She was the pilot of the Soyuz TMA-19, which became the first crew to dock with the station’s Rassvet module.

Joseph Michael "Joe" Acaba is an American educator, hydrogeologist, and NASA astronaut. In May 2004 he became the first person of Puerto Rican heritage to be named as a NASA astronaut candidate, when he was selected as a member of NASA Astronaut Training Group 19. He completed his training on February 10, 2006 and was assigned to STS-119, which flew from March 15 to March 28, 2009 to deliver the final set of solar arrays to the International Space Station. Acaba served as a Flight Engineer aboard the International Space Station, having launched on May 15, 2012. He arrived at the space station on May 17 and returned to Earth on September 17, 2012. Acaba returned to the International Space Station in 2017 as a member of Expedition 53/54.

Dorothy Marie "Dottie" Metcalf-Lindenburger is a former American astronaut. She married Jason Metcalf-Lindenburger, a seventh grade teacher at the time, in 2000, and they have 1 daughter together. She was a science teacher at Hudson's Bay High School in Vancouver, Washington when she was selected in 2004 as an Educator Mission Specialist. She was the first Space Camp alumna to become an astronaut.

Jeanette Jo Epps is an American aerospace engineer and NASA astronaut.